They were divided into control group and treated group in random. Treated group was subdivided into 4 groups according to the different charge: 3C, 5C, IOC, 20C(C: coulomb), 3 wells per group.

• 将等量细胞接种于24孔细胞培养板，按随机原则分为对照组和治疗组，治疗组按电量不同又分为四组：3C、5C、10C、20C(C为电量库仑)，每组3孔。