Therefore, the experimental cells were classified into SKOV3/DCC, SKOV3/Neo and SKOV3.By using reverse transcriptase-polymerase chain reaction and immunocytochemistry, the expression of DCC mRNA and its protein were examined.

• 实验分为3组,DCC基因转染组(SKOV3/DCC组)、空载体转染组(SKOV3/Neo组)和空白对照组(SKOV3组)。 RT-PCR技术和免疫组化染色方法检测3组细胞中DCCmRNA和蛋白的表达,并观察细胞集落形成率、生长速度、细胞周期等生物学行为变化。