Then they were transformed into Escherichia coli BL21(DE3) competent cells for expression. The expressed nucleoprotein was purified using Ni2+-NTA Column under denaturing conditions.

• 将重组质粒转化到大肠杆菌BL21(DE3)中进行表达,表达的核蛋白再经Ni2+-NTA亲和层析纯化。