Then the refolding of protein in the ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC) and size-exclusion chromatography (SEC) was studied.

• 所以本文将首先从描述液-固界面的模型入手，研究溶质在液-固界面的吸附行为受到的影响，在此基础上对变性蛋白在离子交换色谱(IEC)，疏水色谱(HIC)及排阻色谱(SEC)中的复性进行研究。