The whole FC cDNA was cloned into pET-28a (+) containing T7 promoter and recombinant expression plasmid pET-FC was constructed. The recombinant plasmid was transformed into E. coli BL21(DE3).

• 将分段克隆得到的两段FC片段经相应酶切后 ,与含T7启动子的质粒 pET 2 8a(+)在一个体系中作连接反应 ,构建表达质粒pET2 8a FC ,转化大肠杆菌BL2 1(DE3) ,筛选表达菌株。