The use of low-strength soft initiation medium (1/4MS+2% sucrose+0.5% agar,supplemented with 0.5mg/L BAP and O.05mg/L IBA) was effective in preventing browning of the explants.

• 低浓度营养成份的软质启动培养基（1/4MS＋2%25蔗糖＋0.；5%25琼脂，附加0