The target protein can be in a crude extract or a more purified preparation and the monoclonal or polyclonal antibody against this protein is necessary to help us to recognize the antigen.

• 待测蛋白既可以是粗提物也可以经过一定的分离和纯化,另外这项技术的应用需要利用待测蛋白的单克隆或多克隆抗体进行识别。