The shell protein of 5% air-filled microsphere preparation was detected by HPLC,acetate fibre membraneelectrophoresis and semimicro -method.

• 采用高效液相色谱（HPLC）、醋酸纤维膜电泳和半微量定氮法测定5%25声振人血白蛋白空气微球制剂中球壁蛋白的含量。