The results shows that the concentration of Acetylcholinesterase in earthworms was low, it was not steady and its loss during purification process was great.

• 且纯化过程中损失较大，结果欠佳；活化载体－共价结合法固定得到的乙酰胆碱酯酶酶膜活性高，酶不易损失，重现性好。