The relation among esterase isozyme zymogram and district distribution of cultivars and air temperature condition,and application of esterase isozyme in barley breeding were discussed.

• 对湖北大麦酯酶同工酶酶谱类型与地区分布和气温条件的关系，以及酯酶在大麦引种育种生产实践中的应用进行了讨论。