The reduction was conducted in Tris-HCl buffer, and the concentrations of COBE and CHBE and the optical purity of product were determined by capillary GC and HPLC equipped with a chiralcel OB column, respectively. The product was mainly D-(S)-CHBE.

• 实验发现 ；在水相体系中主要生成 D (S) 型产物 ；COBE的转化率及CHBE的收率和光学选择性都比较高 .