The purpose of this study was to establish the animal model of oral submucous fibrosis (OSF) in buccal mucosa of SD rats by submucosa injecting and surface painting aqueous areca nut extracts (AANE).

• 采用粘膜下注射及表面涂布水溶性槟榔提取液（AANE）相结合方法，诱发SD大鼠颊部口腔粘膜下纤维性变（OSF）。