The propagation conditions of PPV, PRV were optimized. The results showed that the PPV-L strain grew better on ST and PK-15 cell lines than on Marc-145 cell lines and the virus titer reached to 106.61TCID50/0.1mL.

• 首先探讨了猪细小病毒(PPV)和猪伪狂犬病病毒(PRV)增殖的6个影响因素，包括细胞系(PK-15、ST、Marc-145、RK、BHK-21)选择、最佳培养基、收毒时间、冻融次数等。