The prokaryotic expression plasmid pET-32a/NAP 5 was constructed successfully. The recombinant was transformed into E. coli BL21(DE3) and expressed by inducing with IPTG and lactose with high efficiency.

• 成功构建了pET-32a/NAP5表达载体,IPTG和乳糖均能诱导目的蛋白在大肠杆菌BL21(DE3)中高效地可溶性表达。