The products of second subtractive hybridization were cloned into the vector of pUC57/T to create a subtractive library. 510 Clones were selected from it and were identified by PCR.

• 将经过两次消减杂交及PCR扩增后的产物经“A一T clone”克隆到pUC57/T载体，进而转染大肠杆菌获得消减文库，共挑选出510个克隆，经PCR鉴定有特异性扩增带且扩增带分子量在200一700bp的克隆有433个，阳性率高达