The positive signals of in situ hybridization appeared in most cells of MCF 7 cells selected by G418.Conclusion: The eukaryotic expression vector of pcDNA3 t PA is successfully constructed and can be expressed in MCF 7 cells.

• 结论:组织型纤溶酶原激活剂真核表达载体构建成功,可在乳癌细胞系MCF7中表达。