The paracrys-talline slime layer become thicker and clearer after fixed with anti-rickettsial serum and stained with ruthenium red. The slime layer disappeared after the extraction of rickettsiae with ether.

• 在固定液中加入相应的免疫血清用钌红细胞化学染色,在立克次体表面可见有肥厚的纤维状物(亚晶格状表面层)围绕的粘液层,经乙醚提取的立克次体标本粘液层消失,而用泛影(?)