The optimal conditions of TLC were as follows:silica gel GF254 as stationary phase, n-hexane-ethyl acetate (5:1, v/v) as mobilephase. The Rf value of hyperforin was 0.41.

• 薄层色谱的最佳条件为:以薄层层析硅胶 GF254 为固定相；以正己烷-乙酸乙酯 (5:1；v/v)为展开剂；贯叶金丝桃素的 Rf值为 0.；41。