The mount of vectors and polybrene, the hatching time for them were optimized through transient transfection of pG-CMV-DRE(2).

• 通过瞬时转染，在转染了载体pG-CMV-DRE(2)和pG-SV-DRE(2)的细胞体内观察到有报告基因绿色荧光蛋白表达。