The major object of this study is to establish the technique systems of Colt shoot-tips culture. Faotors influencing propagation were optimized and discussed, A relatively optimal culture system for Colt shoot-tips is established.

• 本实验第二部分以樱桃砧木Colt的小茎尖为外殖体进行组织培养体系的建立,对繁殖各个阶段的多种影响因素组合进行了系统研究,初步建立了一套较为完整的樱桃砧木Colt的小茎尖培养体系。