The induced condition, sterilized method, type of hormone and its proportion and weathering as well, and culture medium of rooting of tissue culture with Milpa Alta have been explored through the study.

• 通过研究探索出米邦塔仙人掌组织培养的诱导条件、消毒方法、激素的种类和配比以及分化、生根的培养基。