The hydrolysis of ester bonds produced the corresponding carboxylic and phosphatic acid.The acidic groups provide the negative charge that could immobilize cyt c by the electrostatic attraction.

• 酯类官能基化的中孔洞材料可以进一步的经由水解得到酸基，由于酸性官能基在中性条件下提供充分的负电荷，带正电荷的酵素可以经由静电引力被固定化于中孔洞材料中。