The hemagglutinin protein(HA) gene of avian influenza virus was amplified from pVAX1-HA by PCR and sub-cloned into pcDNA3.1(+) vector. HA-CpG gene was also cloned into the pcDNA3.1(+).

• 将从重组质粒pVAX1-HA中扩增的HA基因和从pVAX1-HA-CpG中切出的HA-CpG基因分别克隆入pcDNA3.；1(+)载体中；获得重组质粒pcDNA3