The gross observation, light microscopy, electron transmission microscopy, intravital microscopy and laser Doppler flowmetry were conducted on the combs at time of 5?min, 10?min, 20?min and d 1,d 3 after irradiation.

• 分别于照光5min后即刻、照光10min后即刻、照光20min后即刻、照光20min后第1天、照光20min后第3天分别取6只鸡冠行肉眼、活体显微镜、激光多普勒血流图、光镜和电镜检查,观察鸡冠激光照射处皮肤微循环结构和血流状态的动态变化。