The enzyme was purified by batch-wise adsorption and elution on DEAE-cellulose, Butyl-Toyopearl 650M hydrophobicchromatography and Sephadex G-100 gel filtration chromatography, The purityof the enzyme was increased by 66.8-fold.

• 脂酶提纯时；采用DEAE-纤维素搅拌吸附与洗脱、Bulyl-Toyopearl650 M疏水层析及交联葡聚糖G-100凝胶过滤层析工艺；可提高酶纯度66.；8倍。