The eluates equal to Rf of AF and AFG were isolated with silica gel column,the eluting solvents were n-BuOH:AcOH:H_2O(2:1:1), The solutions containing AF and AFG were respectively combined, concentrated.

• 采用反复聚丙烯酰胺(Bio-gel)柱层析从合成物中分离精氨酸衍生物AF、AFG，作为从人参果中分离AF、AFG的标准品。