The crude protein was obtained by extracting the leaves of Blechnum orientale with PBS and precipitating with (NH 4) 2SO 4. The crude protein was purified by Sephadex G-100 filtration after treatment through active carbon column.

• 乌毛蕨的叶片经pbs抽提和硫酸铵沉淀 ；得到的粗蛋白经活性碳柱处理后 ；在分子筛层析柱SephadexG -10 0上纯化得到一种凝集素 (BlechnumOrientaleLectin ；简称BOL) .