The completed VP 2 gene was amplified from PPV RFDNA by PCR method. Then the products were cloned into pMD18-T vector and the sequence was determined.

• 利用PCR技术从猪细小病毒的RFDNA模板中扩增了含有VP2 全基因的 2 0kb的基因片段 ,将PCR产物克隆至 pMD18 T载体 ,利用双脱氧末端终止法测定了VP2 全基因的核苷酸序列。