The column was hypersil BDS(4mm x 250mm). The mobile phase was 0. 1% triethylamine(adjusted to pH 3.2 with phosphoric acid)-acetonitrile(85:15). The detective wavelength was 256nm and the flow rate was 1ml/min.

• 色谱柱为Hypersil BDS(4mm×250mm)；流动相为0.；1%25三乙胺(用磷酸调pH至3