The cis-vinylic linkage and the bulky side groups were considered to be the major factor to produce such inaccessible fluorophore.

• 而在不存在大体积的苯取代基时，顺式构型的存在应该是产生这种不可接触发色团的主要因素。而链间聚集以及季胺化不完全等其它因素对荧光猝灭行为的影响则较小。