The changes of [Ca~(2+)]i and CaM mRNA expression were detected by fluorometric method and RT-PCR. The result showed that ATO induced MDCC-MSB1 apoptosis by the increase of [Ca~(2+)]i and disequilibrium of calcium homeostasis.

• 应用Fura-2/AM荧光法和RT-PCR方法分别检测细胞内[Ca~(2+)]i的变化和CaMmRNA表达水平,结果表明As_2O_3可通过引起MDCC-MSB1细胞内[Ca~(2+)]i增加,钙稳态失衡机制而诱导细胞凋亡。