The VH and VL genes were inserted into a plasmid which contains a linker sequence for constructing scFv gene.The new vector named pTC scFv.

• 将其和先前克隆的轻链基因分别插入到一个含有连接VH和VL基因的连接序列的质粒之中，构建成单链抗体（scFv）基因的表达载体pTCscFv。