The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR.

• 在此基础上，将含有多克隆位点和编码NIa蛋白酶识别位点的Adaptor通过PCR介导插入到芜菁花叶病毒CDN1株系全长cDNA的NIb与CP区之间的NIa蛋白酶识别位点之后，将芜菁花叶病毒CDN1株系全长cDNA侵染性克隆pVIR95T改造成一个可利用的基因插入型表达载体pVIR95TM。