The Sox gene of Rana rugulosa Wiegmann was amplified by using the technique of PCR with a pair of primers which specially amplify the conserved motif (HMG-box) of human SRY gene.

• 采用PCR技术，以特异扩增人SRY基因HMG-box保守区的一对特异引物扩增了虎纹蛙Sox基因，优化了扩增条件。