The HPLC method with a good result of separation has been developed for the determination of gentamicin intermediate ingredients, inclusive of shake flask, fermentation broth and desorption solution.

• 应用HPLC法测定庆大霉素中间体，包括摇瓶、发酵液、解吸液等的组份分析，分离效果良好，具有使用价值。