The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.

• 采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。