The AVA gene with and without intron were inserted downstream of 35S promoter into a vetor pBI121 to get pBI121AVA-GUS and pBI121AVAc-GUS vetor respectively.

• 采用反向PCR技术获得了该基因的编码区克隆。 分别构建含有内含子和不含内含子的AVA基因植物表达载体pBI121AVA-GUS和pBI121AVAc-GUS,通过根癌农杆菌介导法转化烟草。