Somatostatin DNA vaccine plasmid pCHSS(pCDNA/HBsAg/SS)has been constructed and expressed in COS cells at a high level. About 27% fusion protein is secreted outside the cells by detecting the HBsAg label.

• 构建了生长抑素DNA疫苗质粒 pCHSS(pCDNA/HBsAg/SS) ,并在COS细胞中的获得较高水平的瞬时表达 ,用HBsAgElisa检测试剂盒跟踪发现 ,约有 2 7%25的融合蛋白分泌到细胞外 ,说明SS的插入没有影响HBsAg的免疫反应性和分泌表达。