SPF chick embryos at the age of eight days were inoculated with avian encephalomyelitis virus (AEV) and then hatched in the incubator.

• 摘要通过卵黄囊接种法，将禽脑脊髓炎病毒接种到8日龄SPF鸡胚内，继续在孵化箱内孵化。