SDS-PAGE results showed that there was a clear target protein band in Mut+ recombinant supernatant after 48 hours of culturing, while a faint band only in Muts recombinant after 72 hours.

• 结果表明，诱导培养48小时后，Mut~+重组菌株表达产物在SDS-PAGE胶上显现出清晰的目的蛋白带，而Mut~s重组菌株培养72小时才能显示微弱的目的带；