SDS PAGE and Dot blot indicated that this 12kD protein was PF4.ConclusionUsing SZ 95 sepharose 4B affinity chromatography to purify PF4 is an efficient method to prepare PF4 with high biological activity.

• 结论用SZ 95 Sepharose 4B亲和色谱柱纯化的PF4产品得率高、纯度高、活性好