RhoA activity was measured by pull down assay, and the activity of ROK was evaluated using antibody against phosphorylated MBS.

• Pulldown方法检测用RhoA活性,ROK活性用其底物MBS的磷酸化来表示,总RhoA、ROK及磷酸化MBS蛋白表达用免疫印迹法测定,用ELISA法检测细胞因子水平。