Reverse Transcription (RT) PCR. The mRNA expressions of cyclin D1, cyclin B1, CDK4, cdc2 and p21 were determined by RT-PCR semi-quantitatively.

• 逆转录PCR(RT-PCR)：应用RT-PCR半定量检测细胞cyclin D1、cyclin B1、CDK4、CDK1、p21的mRNA表达。