Results:1 .Establishing a method to separate gene fragments, combining with the RD technique with DNA silver staining, the results showed that in the PAGE gel, every group contained 7-10 clear DNA bands on average.

• 将DNA银染技术与RD技术结合起来，分离基因片段，在PAGE胶上可发现，平均每组有7-10条DNA带，在日光灯下，带型清楚，可以快速分离EST片段，扩增后无杂带产生，直接可以用于载体克隆。