Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively.

• 结果3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%25左右,Fab抗体柱纯化的Fab抗体的纯度为95%25,但这两种亲和柱的目的蛋白收率都不高,分别为35%25、55%25。