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Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome.
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细胞核载体PB-adw、PBG-adw均采用农杆菌介导法将adw导入莴苣,细胞核转化获得了生长良好的抗性莴苣植株,经PCR、PCR-Southern、southern斑点杂交分析证实,adw基因已整合到莴苣基因组中。
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