Recombinant plasmid, pBYBl, with full length cry1Aa14 gene was constructed and transformed into a Bt acrystalliferous mutant strain HD73 cry.

• cry1Aa14全长基因插入Bt表达载体，获得了重组表达质粒pBYB1，转化大肠杆菌SCS110和Bt无晶体突变株HD73cry~-，经过抗性筛选、DNA酶切分析和PCR检测，证实转化成功。