Recombinant human cTnI protein was used as antigen to immunize BALB/c mice. Monoclonal anti-bodies against cTnI were prepared by normal hybridoma technology. The relative affinity of mAbs was determined by ELISA.

• 以基因重组的cTnI蛋白为抗原,常规方法免疫BALB/c小鼠,取其脾细胞与NS1细胞融合,获得稳定分泌cTnImAb的杂交瘤细胞株,ELISA检测mAb的相对亲和力;