Recombinant Bacillus thuringiensis strain Btt-WF45 was obtained by electroporating the plasmid containing cyt1Aa into Bacillus thuringiensis subsp. Tenebrionis(Btt)strain which is toxic to coleopterous insects.

• 将含苏云金芽孢杆菌以色列亚种(Bacillus thuringiensis subsp.；israelensis；简称Bti)cyt1Aa基因的质粒电激转化至对鞘翅目害虫有专一活性的Bt拟步甲亚种(Bacillus thuringiensis subsp