Pseudorabies viruses (PRV) were replicated in cells of hamster origin (BHK 21 ) and then the PRV DNA extracted.

• 在BHK21细胞中增殖伪狂犬病病毒（PRV），提纯PRVDNA，选编码特异性糖蛋白gp50基因中的262bp片段进行PCR扩增。